Denominazione

Mass Spectrometry Core Facility

Core Facility Coordinator

Gianluca Damonte

Description

The Center of Excellence for Biomedical Research (CEBR) mass spectrometry core facility mainly concerns the structural characterization of biological molecules such as peptides, PNA, nucleotide and nucleotide-analog molecules with potential anticancer and antiviral activity as well as the structural characterization of proteins, oligonucleotides, carbohydrates, lipids and molecules with terpene structure by means of gas and liquid chromatography coupled to mass spectrometry. These analysis allow, by mean of modern techniques, the chromatographic separation of analites in complex mixtures as biological fluids and tissues. The possibility of using the mass spectrometer as a detector coupled "on-line" with separative techniques allows the realization of molecular studies ranging from the direct analysis of protein variants responsible for serious genetic diseases, and proteomic analysis (by means of "fingerprinting" or MS/MS) or, finally, the qualitative and quantitative pharmacokinetics "in vitro" and "in vivo" of pharmacologically active molecules. One of the Mass Spectrometry Core Facility activities concerns the use of molecules with peptide and/or modified oligonucleotides structure in the antisense and/or antigen therapy. The activity of the Facility is also devoted to the structural characterization of synthetic intermediates and products for their potential use in the pharmacological treatment of various diseases including viral infections, cancer or inflammatory diseases.
The CEBR Mass Spectrometry Core Facility is equipped with different instrumentation, reported below:

  • Binary LC System working at analytical, micro and nano fluxes coupled with a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer equipped with nanospray, electrospray and MALDI ion sources.
  • 1100 µLC system coupled with Ion Trap 1100 XCT mass spectrometer equipped with an electrospray ion source (Agilent Tecnologies).
  • 1260 Infinity Binary µLC system (Agilent Tecnologies) coupled with LCQ Fleet Ion Trap Mass Spectrometer equipped with an electrospray ion source (Thermo Scientific).
  • 1260 Infinity Binary LC system coupled with 1100 MSD series single quadrupole mass spectrometer equipped equipped with an electrospray ion source (Agilent Tecnologies).
  • Gas chromatograph (Hewlett Packard 5890 Engine) coupled with mass spectrometers equipped with an electron impact (EI) interface (Hewlett Packard 5970 Engine).
  • 1260 Infinity Binary LC system equipped with Diode Array and Fluorescence Detector (Agilent Tecnologies).
  • 1260 Infinity Preparative scale Purification System equipped with Diode Array Detector (Agilent Tecnologies).
  • Waters HPLC 1525 Binary HPLC Pump and 2998 Photodiode Array Detector (Waters Corporation).

Rate table

Center of Excellence for Biomedical Research.
Mass Spectrometry Core Facility
Rate table, for Public Institutions and Companies

  • Qualitative analysis using GC/MS € 60.00 *
  • Quantitative analysis using GC/MS €/hour 100.00 *
  • Interpretation of mass spectra obtained analyzing samples by mean of GC/MS or LC/MS and structural characterization related €/hour 120.00
  • Qualitative analysis by mean of direct infusion DIA LC/MS ESI € 50.00 *
  • Qualitative analysis by mean of HPLC/ESI/MS € 120.00 *
  • Quantitative analysis by mean of HPLC/ESI/MS €/hour 100.00 *
  • Development of chromatographic (HPLC) and spectrometric (HPLC/MS and GC/MS) methods € / hour 100.00 *
  • Separation in preparative HPLC (including scaling-up method) €/hour 100.00 *

*Any costs relative to the preparation of the samples will be arranged from time to time depending on the complexity of matrices and samples.